Longevity & Metabolic Resilience Protocol

Protocol Overview

This advanced longevity protocol combines three mitochondrial-derived peptides (MDPs) that target fundamental aging mechanisms: MOTS-c for metabolic optimization, Humanin for cellular protection, and Epitalon for telomere maintenance. Together they address mitochondrial function, cellular senescence, and age-related metabolic decline.

Targeted Aging Mechanisms

• Mitochondrial dysfunction - Declining cellular energy production
• Metabolic decline - Insulin resistance, reduced fat oxidation
• Telomere shortening - Cellular aging and replicative senescence
• Cellular stress - Oxidative damage and inflammation
• Circadian disruption - Age-related sleep and hormone changes

Expected Benefits

• Enhanced energy and vitality
• Improved metabolic function
• Better insulin sensitivity
• Increased exercise capacity
• Improved sleep quality
• Enhanced cognitive function
• Potential lifespan extension (based on animal studies)

Understanding the Peptides

MOTS-c: The Metabolic Optimizer

MOTS-c is a 16-amino acid peptide encoded in mitochondrial DNA. It acts as a metabolic regulator, improving insulin sensitivity and enhancing fat oxidation.

Key Mechanisms:

• AMPK activation: Master metabolic regulator
• Insulin sensitivity: Improves glucose uptake
• Fat oxidation: Enhances mitochondrial fat burning
• Exercise mimetic: Produces exercise-like metabolic effects
• Longevity pathways: Activates sirtuins and other longevity genes

Humanin: The Cellular Protector

Humanin is a 24-amino acid peptide that protects cells from various stressors including oxidative damage, inflammation, and apoptosis.

Key Mechanisms:

• Neuroprotection: Protects against Alzheimer's pathology
• Metabolic protection: Improves insulin sensitivity
• Anti-apoptotic: Prevents premature cell death
• Anti-inflammatory: Reduces systemic inflammation
• Cardiovascular protection: Protects heart and blood vessels

Epitalon: The Telomere Lengthener

Epitalon is a synthetic peptide that activates telomerase, the enzyme responsible for maintaining telomere length.

Key Mechanisms:

• Telomerase activation: May lengthen telomeres
• Circadian regulation: Normalizes melatonin production
• Antioxidant effects: Reduces oxidative stress
• Hormone regulation: Normalizes age-related hormone changes
• Lifespan extension: Increased lifespan in animal studies

Dosing Protocol

MOTS-c Dosing

Dose: 5-10 mg per injection

Frequency: 2-3 times per week

Route: Subcutaneous or intramuscular

Timing: Morning or pre-workout

Humanin Dosing

Dose: 2-5 mg per injection

Frequency: 2-3 times per week

Route: Subcutaneous

Timing: Can be combined with MOTS-c

Epitalon Dosing

Dose: 5-10 mg per injection

Frequency: Daily for 10-20 days

Route: Subcutaneous

Timing: Evening (supports melatonin)

Cycling: 10-20 day cycles, 2-4 times per year

Combined Protocol Schedule

Phase 1: Foundation (Weeks 1-12)

• Monday/Wednesday/Friday: MOTS-c 5-10 mg + Humanin 2-5 mg
• Daily for 10 days: Epitalon 5-10 mg (start week 1)
• Continue MOTS-c + Humanin after Epitalon cycle ends

Phase 2: Maintenance (Weeks 13-24)

• Monday/Thursday: MOTS-c 5-10 mg + Humanin 2-5 mg
• Epitalon: Repeat 10-day cycle at week 16

Long-term Strategy

• Continue MOTS-c + Humanin 2-3x weekly indefinitely
• Epitalon cycles 2-4 times per year
• Take 4-week breaks every 6 months

Expected Results Timeline

Weeks 1-4: Initial Metabolic Shift

• Increased energy levels
• Improved exercise performance
• Better sleep quality (Epitalon)
• Enhanced mental clarity

Weeks 5-12: Metabolic Optimization

• Improved body composition
• Better insulin sensitivity
• Enhanced fat oxidation
• Increased vitality
• Improved recovery

Weeks 13-24: Long-term Benefits

• Sustained metabolic improvements
• Potential telomere lengthening
• Enhanced healthspan markers
• Better stress resilience
• Improved overall well-being

Lifestyle Optimization

Exercise Integration

• HIIT training: 2-3x weekly synergizes with MOTS-c
• Resistance training: 3-4x weekly for muscle maintenance
• Zone 2 cardio: 30-45 minutes, 2-3x weekly
• Recovery: Adequate rest between sessions

Dietary Strategies

• Time-restricted eating: 16:8 or 18:6 fasting
• Mediterranean diet: Anti-inflammatory, longevity-promoting
• Protein optimization: 1.6-2.0 g/kg body weight
• Polyphenols: Berries, green tea, dark chocolate

Supplements

• NAD+ precursors: NMN or NR 250-500mg daily
• Resveratrol: 500mg daily
• Omega-3s: 2-4g EPA+DHA daily
• Vitamin D: 2,000-5,000 IU daily
• Magnesium: 400mg daily

Monitoring and Biomarkers

Baseline Testing

• Comprehensive metabolic panel
• Lipid panel
• HbA1c and fasting insulin
• hs-CRP (inflammation)
• IGF-1
• Hormone panel
• Body composition (DEXA scan)

Progress Monitoring (Every 12 weeks)

• Metabolic markers
• Body composition
• Exercise performance metrics
• Sleep quality tracking
• Subjective well-being scores

Cost Analysis

24-Week Protocol Cost

• MOTS-c: ~$400-800
• Humanin: ~$300-600
• Epitalon (2 cycles): ~$200-400
• Total: $900-1,800 for 24 weeks
• Monthly cost: $150-300